Nordmark Collagenase NB Product Technical

Crescent Chemical Co., Inc. supplies Nordmark Collagenase NB obtained from Clostridium histolyticum.
Collagenases obtained from Clostridium histolyticum are enzymes that break the peptide bond in collagen. Clostridium histolyticum secretes Collagenase Class I and II, Neutral Protease and Clostripain.
These four enzymes work together to dissociate the extracellular matrix for cell isolation from tissue for clinical and research applications.
Scroll down for Crescent's line of Nordmark Collagenase NB and Product Notes.

New Nordmark Collagenase NB 7D Gentle Grade

Catalog #17474.01 500 mg

Catalog #17474.02 1 gm

Catalog #17474.03 5 gm

Class I and II Collagenase and reduced trypsin like activities

PZ activity ≥ 0.2 U/mg Lyphosilate

Trypsin like activity (BAEE) ≤ 0.2 U/mg Lyphosilate

Ratio Collagenase Trypsin ≥ 3.0

Used for a variety of applications where gentle conditions are beneficial.

Nordmark Collagenase AF-1 GMP Grade (The World's First Animal-free Collagenase)

Cat. No. 17457.01

≥ 2,000 U(PZ)

Completely Animal-free

Chromatographically highly purified

Class l and ll collagenases

Very low levels of endotoxins

PZ activity (Wünsch) ≥ 3.00 U/mg

Isolation of especially sensitive cells including pancreatic islets, liver and dental pulp cells

Click here for Nordmark Collagenase AF-1 GMP Grade Product Notes

Nordmark Neutral Protease AF GMP Grade (The World's First Animal-free Neutral Protease)

Cat No. 30306.01

≥ 100 U(DMC)

Chromatographically purified and largely free from collagenolytic activity (PZ U) as well as endotoxins

Absent of any animal-derived ingredients and thorough microbial analysis provide the highest possible safety

Activity (DMC assay) ≥ 0.50 U/mg

Click here for Nordmark Neutral Protease AF GMP Grade Product Notes

Nordmark Collagenase NB 1 GMP Grade

Cat. 17452.01

2,000 U(PZ)

Manufactured according to Intl. cGMP guidelines

PZ activity (Wünsch) ≥ 3.00 U/mg

Isolation of pancreatic islets for transplantation into humans

Use in combination with Nordmark Neutral Protease NB GMP Grade

Click here for Nordmark Collagenase NB 1 GMP Grade Product Notes

Nordmark Neutral Protease NB GMP Grade

Cat. No. 17452.01

≥ 100 U(DMC)

Chromatographically purified and largely free from collagenolytic activity (PZ U) and endotoxins

Activity (DMC assay) ≥ 0.50 U/mg

Cell isolation in combination with Collagenase NB 1 GMP Grade or Collagenase NB 6 GMP Grade from various tissue types for clinical applications.

Click here for Nordmark Neutral Protease NB GMP Grade Product Notes

Nordmark Collagenase NB 1 Premium Grade

Cat. No. 17455.03

≥ 2,000 U(PZ)

Chromatographically highly purified

Class l and ll collagenases

Use in combination with Nordmark Neutral Protease NB

PZ activity * ≥ 3.0 U/mg lyophilisate

Largely free of clostripain, trypsin-like proteases

Endotoxins ≥ 10 EU/mg lyophilisate

Collagen characterization

Isolation of pancreatic islets

Click here for Nordmark Collagenase NB 1 Premium Grade Product Notes

Nordmark Neutral Protease NB

Cat. No. 30301.11/ 50 U (DMC)

Cat. No. 30301.12/ 100 U (DMC)

Chromatographically purified neutral protease.

Activity (DMC assay) ≥ 0.50 U/mg

Cell isolation in combination with Nordmark Collagenase NB

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Nordmark Neutral Protease NB High Active Grade

Cat. No. 30302.02

80 U(DMC)

Chromatographically purified and contains an increased proteolytic activity

Largely free from collagenolytic activity (PZ U)

Endotoxin level is very low

Activity (DMC assay) ≥ 4.00 U/mg

Endotoxin ≥ 50.0 EU/mg

Cell isolation in combination with Nordmark Collagenase NB product

Click here for Nordmark Neutral Protease NB High Active Grade Product Notes

Nordmark Collagenase NB 4 Standard Grade

Cat. No. 17454.01/ 1 g

Cat. No. 17454.02/ 500 mg

Cat. No. 17454.03/ 5 g

Class l and ll collagenases and a balanced ratio of proteolytic activities

PZ activity* ≥ 0.1 U/mg lyophilisate

Isolation of various cell types including neuronal and endothelial cells, chondrocytes, adipocytes epithelial cells, stem cells, gangliocytes and periodontal cells

Click here for Nordmark Collagenase NB 4 Standard Grade Product Notes

Nordmark Collagenase NB 4G Proved Grade

Cat. No. 17465.01/ 500 mg

Cat. No. 17465.02// 1 g

Cat. No. 17465.03/ 5 g

Class l and ll collagenases as well as a balanced mix of neutral protease, clostripain, and tryptic activities

PZ act.* ≥ 0.18 U/mg lyophilisate

Fuction tested for cell isolation

Isolation of adipocytes, fibroblasts hepatocytes, chondrocytes, stem cells, frog oocytes, mastocytes and tumor cells

Click here for Nordmark Collagenase NB 4G Proved Grade Product Notes

Nordmark Collagenase NB 5 Sterile Grade

Cat. No. 17459.03 / 1 g

Sterile according to European req. For medicinal prods. (Pharm. Eur.)

PZ activity (Wünsch): ≥ 0.10 U/mg

Applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.

Click here for Nordmark Collagenase NB 5 Sterile Grade Product Notes

Nordmark Collagenase NB 6 GMP Grade

Cat. No. 17458.03/ 1 g

Cat. No. 17458.04/ 100 mg

Aseptic production according to European req. for medicinal prods.(Pharm. Eur.)

Manufactured according to Intl. cGMP guidelines

PZ activity (Wünsch): ≥ 0.100 U/mg

Applicable to clinical tissue dissociation such as isolation of human ADSCs, stem cells, chondrocytes, fibroblasts, neuronal, endothelial hepatocytes, cardiomyocytes, tumor, muscle, epithelial, gangliocytes, oocytes and periodontal cells.

Click here for Nordmark Collagenase NB 6 GMP Product Notes

Nordmark Collagenase NB 8 Broad Range

Cat. No. 17456.01/ 250 mg

Cat. No. 17456.02/ 1 g

Class l and ll collagenases and reduced levels of clostripain, trypsin-like activities and neutral protease

PZ act.* ≥ 0.9 U/mg lyophilisate

Endotoxins ≥ 100 EU/mg lyophilisate

Isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic islets tumor and thyroid cells.

Click here for Nordmark Collagenase NB 8 Broad Range Grade Product Notes

Nordmark Clostripain NB
Used in combination with other Collagenases and Neutral Proteases to improve your isolation results from human and animal tissue.

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Collagenase N, Anti Collagenase, Collagenase Calculator, Drug Master File (DMF) and Frequently asked questions (FAQ):

Collagenase NReference Content / ≥ 50 mgCat. No. 17463

Class l and ll collagenases and other proteolytic enzymes

Collagenase activity is regularly reevaluated

Reference substance for enzyme activity determination.

Collagenase NReference Identity / ≥ 50 mgCat. No. 17464

Class I and ll collagenases and other proteolytic enzymes

Reference substance for SDS-PAGE analysis, for incoming goods inspection according to cGMP

Anti-CollagenaseCat. No. 58050 / 100 μ

Specifically against collagenases from C. histolyticum

lgG fraction purified by Protein G affinity chromatography

Use in ELISA and Western Blot experiments

Immunostaining of tissue

Analysis of residual collagenase impurities in cell preparations

Anti-Collagenase HRPCat. No. 58051 / 100 μ

Specifically against collagenases from C. histolyticum

lgG fraction purified by affinity chromatography

Conjugated with horseradish

Peroxidase

Use in ELISA and Western Blot experiments

Immunostaining of tissue

Analysis of residual collagenase impurities in cell preparations

* PZ activity acc. to Wϋnsch, measured at 25 ° C.